PHA, arcelins, and Alpha-AIL fail to inhibit amylase to a large degree due to steric hindrance from their looped structures, and only Alpha-AI1 shows effective amylase binding abilities in vivo in mammals.

Alpha-AI1 seems to have highly unpredictable effects on in vivo carbohydrate metabolism as the activity of the binding process is influenced highly by pH, temperature (when away from optimum pH) and coingestion with particular ions as Nitrate, chloride, bromide, iodide, thiocyanate, and calcium ions all increase the ability for Alpha-AI1 to bind to amylase, whereas potassium, magnesium, sulfate and sodium showed no effect. As well as other indirect effects like an increased secretion of amylase from the pancreas in response to slower intestinal transit time

AmalysIn is a brand name for a specific processed extract of Phaseolus Vulgaris. Extracts of Phaseolus vulgaris can result in a very high phytohaemagglutinin (PHA) content, which would reduce the high safety threshold of white kidney bean extract significantly. They also have been reported to have much lower intestinal action on the amylase enzyme.

Via inhibition of Alpha-amylase, Alpha-AI1 prevents long chain carbohydrates from being taken up in the small intestine for nutritional value to the human.

This causes said starches to pass through to the colon where they have the potential to be fermented by bacteria. In this manner, Alpha-AI1 can make ingested carbohydrates act like a probiotic.

When fermented, these carbohydrates can release short-chain fatty acids including propionic acid, butyric acid, and acetic acid. These short chain fatty acids are thought to be involved in reducing the risk of colon cancer

AmalysIn to be effective in inducing starch malabsorption and thus induce weight loss by ways of mimicking a caloric deficit. Human studies all show benefit in supplementation with the highest starch containing meal, although the degree of benefit seems highly variable.

AmalysIn is not recommended as a first-line fat burner due to its inability to induce fat loss, but can be used as a band-aid supplement to a subpar diet like most calorie blockers.

AmalysIn [White Kidney Bean extract, is a dietary carbohydrate blocker similar to Chlorogenic acid and is sometimes used alongside carb containing meals to reduce the absorption of carbohydrates