Withaferin – A is a natural compound isolated from the medicinal plant Withania somnifera, an important ingredient in Indian Ayurvedic medicine, and has been widely studied for its anti-inflammatory properties. Recently, studies characterizing the biological activities of withaferin - A have expanded to include the determination of its anticancer properties. Withaferin - A was initially reported to inhibit cell growth and induce apoptosis in in vitro and in vivo models of breast cancer.
Subsequent studies of the anticancer activity of withaferin - A in colon cancer cells demonstrated its ability to modulate Notch1.
Withaferin – A treatment significantly inhibited Notch1 signalling as measured by downregulation of pathway target genes HES1 and HEY1.
In addition, it downregulated numerous prosurvival pathways such as the Akt/NF-κB/Bcl-2 signaling network and the mTOR pathway components pS6K and p4E-BP1. Finally, withaferin- Aactivated c-Jun N-terminal kinase-mediated apoptosis in colon cancer cells.
By using isogenic cancer cells with/without telomerase, Withaferin – A caused stronger cytotoxicity to ALT cells. It was associated with inhibition of ALT-associated promyelocytic leukemia nuclear bodies, an established marker of ALT.
Comparative analyses of telomerase positive and ALT cells revealed that Withaferin – A caused stronger telomere dysfunction and upregulation of DNA damage response in ALT cells. Molecular computational and experimental analyses revealed that Withaferin – A led to Myc-Mad mediated transcriptional suppression of NBS-1, an MRN complex protein that is an essential component of the ALT mechanism. The results suggest that Withaferin –A could be a new candidate drug for ALT cancers.
| Product Name | ASHWAGANDHA EXTRACT 50% [Withaferin-A] |
| Botanical Name | Withania somnifera |
| Plant part | Leaves |
| Standardization | Withaferin-A |
| S. No. | Parameters | Specification | Method |
| Physical | |||
| 1 | Description | Off white to gray color Powder | Visual |
| 2 | Identification | To comply by TLC | ALPL/QC/STP-IN/008 |
| 3 | Loss on drying | NMT 5.0% w/w | USP <731> |
| 4 | Residue on ignition | NMT 7.0% w/w | USP <281> |
| 5 | Tapped bulk density | 0.20– 0.60g/ml | USP <616> |
| Sieve Test (passes through) | |||
| 6 | # 40 mesh | NLT 100.0% w/w | USP <786> |
| # 60 mesh | NLT 90.0% w/w | ||
| Chemical | |||
| 7 | Assay by HPLC Content of Withaferin-A |
NLT 50.0% w/w on dry basis | ALPL/QC/STP-IN/062 |
| Others | |||
| 8 | Heavy Metals | ||
| Lead | NMT 5.0ppm | ICP-OES | |
| Arsenic | NMT 1.0ppm | ICP-OES | |
| Cadmium | NMT 0.3ppm | ICP-OES | |
| Mercury | NMT 0.1ppm | ICP-OES | |
| 9 | Residual Solvents | Should comply by ICH/USP | USP<467> |
| Microbiological Profile | |||
| 10 | Total plate count | NMT 5000cfu/g | USP<2021> |
| Yeast and moulds count | NMT 100cfu/g | USP<2021> | |
| Escherichia coli | Absent/10g | USP<2022> | |
| Salmonella | Absent/10g | USP<2022> | |
| Staphylococcus aureus | Absent/10g | USP<2022> | |
| Pseudomonas aeruginosa | Absent/10g | USP<62> | |
| Enterobacteriaceae | Absent /10g | USP <2021> | |
| Additional Information | |||
| Sanitizing treatment | Non- irradiated and not treated with ETO | ||
| Genetic Modification Status | GMO free | ||
| Storage condition | Store at room temperature | ||
| Manufactured by | Aura Leafs Labs Private Limited Plot #10, Sy.No-230 to 243, Synergy Square-I, MN Park, Genome Valley, Turkapally Village, Shamirpet Mandal, Medchal District, Telangana, Hyderabad-500101, India. |
||